Mouse Interleukin-27 subunit beta (EBI3) ELISA Kit | KTE71223

Mouse Interleukin-27 subunit beta (EBI3) ELISA Kit | KTE71223 | Gentaur UK, US & Europe Distribution

Application: This Mouse Interleukin-27 subunit beta (EBI3) ELISA Kit employs a two-site sandwich ELISA to quantitate EBI3 in samples. An antibody specific for EBI3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyEBI3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EBI3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EBI3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Interleukin-27 subunit beta microplate
• Mouse Interleukin-27 subunit beta standard
• Mouse Interleukin-27 subunit beta detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Interleukin-27 subunit beta (EBI3) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse EBI3. No significant cross-reactivity or interference between Mouse EBI3 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Epstein-Barr virus induced gene 3was identified by the induction of its expression in B lymphocytes by Epstein-Barr virus infection. The protein encoded by this gene is a secreted glycoprotein, which is a member of the hematopoietin receptor family related to the p40 subunit of interleukin 12 (IL-12) . It plays a role in regulating cell-mediated immune responses.EBI3 is a subunit in 2 distinct heterodimeric cytokines: interleukin-27 (IL27) and IL35. IL27 is composed of p28 (IL27) and EBI3. IL27 can trigger signaling in T cells, B cells, and myeloid cells. IL35, an inhibitory cytokine involved in regulatory T-cell function, is composed of EBI3 and the p35 subunit of IL12.Interleukin-27 (IL-27) is composed of two subunits, Epstein-Barr virus (EBV) -induced gene 3 (EBI3) (IL-27B) and IL27-p28 (known as IL-30) .

Alternative Names: EBI3; IL27B; Epstein-Barr virus induced gene 3; cytokine receptor; interleukin-27 subunit beta

Search name: EBI3; IL27B; Epstein-Barr virus induced gene 3; cytokine receptor; interleukin-27 subunit beta

Tag: EBI3