Mouse KLK6 (Kallikrein 6) ELISA Kit | ELK4690

Mouse KLK6 (Kallikrein 6) ELISA Kit | ELK4690 | Gentaur UK, US & Europe Distribution

Alternative Names: ZYME; Bssp; Klk7; Neurosin, Zyme; PRSS18; PRSS9; SP59; HK6; Protease M; Serine protease 18

Species: Mouse

Assay Type: Sandwich

Sensitivity: 13.4 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: serum, plasma, cerebrospinal fluid, breast milk and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase;Tumor immunity;Neuro science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kallikrein 6 (KLK6) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Kallikrein 6 (KLK6) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kallikrein 6 (KLK6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Kallikrein 6 (KLK6) in the samples is then determined by comparing the OD of the samples to the standard curve.