Mouse Lipoxygenase homology domain-containing protein 1 (LOXHD1) ELISA Kit | KTE71471

Mouse Lipoxygenase homology domain-containing protein 1 (LOXHD1) ELISA Kit | KTE71471 | Gentaur UK, US & Europe Distribution

Application: This Mouse Lipoxygenase homology domain-containing protein 1 (LOXHD1) ELISA Kit employs a two-site sandwich ELISA to quantitate LOXHD1 in samples. An antibody specific for LOXHD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLOXHD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LOXHD1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LOXHD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Lipoxygenase homology domain-containing protein 1 microplate
• Mouse Lipoxygenase homology domain-containing protein 1 standard
• Mouse Lipoxygenase homology domain-containing protein 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Lipoxygenase homology domain-containing protein 1 (LOXHD1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse LOXHD1. No significant cross-reactivity or interference between Mouse LOXHD1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: LOXHD1 encodes a highly conserved protein consisting entirely of PLAT (polycystin/lipoxygenase/alpha-toxin) domains, thought to be involved in targeting proteins to the plasma membrane. Studies in mice show that this gene is expressed in the mechanosensory hair cells in the inner ear, and mutations in this gene lead to auditory defects, indicating that this gene is essential for normal hair cell function. Screening of human families segregating deafness identified a mutation in this gene which causes DFNB77, a progressive form of autosomal-recessive nonsyndromic hearing loss (ARNSHL) . Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.Loxhd1 progressively localized to the cytoplasm, and in the adult, Loxhd1 was expressed in hair cells along the length of stereocilia.

Alternative Names: LOXHD1; DFNB77; FLJ32670; LH2D1;

Search name: LOXHD1; DFNB77; FLJ32670; LH2D1;

Tag: LOXHD1