855
Mouse LPCAT4 (Lysophosphatidylcholine Acyltransferase 4) ELISA Kit | ELK6734
- SKU:
- 855-ELK6734-GEN
Description
Mouse LPCAT4 (Lysophosphatidylcholine Acyltransferase 4) ELISA Kit | ELK6734 | Gentaur UK, US & Europe Distribution
Alternative Names: AYTL3; AGPAT7; LPAAT; LPEAT2; Lysophosphatidylethanolamine Acyltransferase 2; Acyltransferase Like 3; 1-Acylglycerol-3-Phosphate O-Acyltransferase 7; Plasmalogen synthase
Species: Mouse
Assay Type: Sandwich
Sensitivity: 0.212 ng/mL
Standard: 40 ng/mL
Detection Range: 0.63-40 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Enzyme & Kinase
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lysophosphatidylcholine Acyltransferase 4 (LPCAT4) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Lysophosphatidylcholine Acyltransferase 4 (LPCAT4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lysophosphatidylcholine Acyltransferase 4 (LPCAT4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lysophosphatidylcholine Acyltransferase 4 (LPCAT4) in the samples is then determined by comparing the OD of the samples to the standard curve.