Mouse Lysophosphatidylcholine acyltransferase 2 (LPCAT2) ELISA Kit | KTE71166

Mouse Lysophosphatidylcholine acyltransferase 2 (LPCAT2) ELISA Kit | KTE71166 | Gentaur UK, US & Europe Distribution

Application: This Mouse Lysophosphatidylcholine acyltransferase 2 (LPCAT2) ELISA Kit employs a two-site sandwich ELISA to quantitate LPCAT2 in samples. An antibody specific for LPCAT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLPCAT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LPCAT2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LPCAT2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Lysophosphatidylcholine acyltransferase 2 microplate
• Mouse Lysophosphatidylcholine acyltransferase 2 standard
• Mouse Lysophosphatidylcholine acyltransferase 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Lysophosphatidylcholine acyltransferase 2 (LPCAT2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse LPCAT2. No significant cross-reactivity or interference between Mouse LPCAT2 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: LPCAT2 encodes a member of the lysophospholipid acyltransferase family. The encoded enzyme may function in two ways: to catalyze the biosynthesis of platelet-activating factor (1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) from 1-O-alkyl-sn-glycero-3-phosphocholine, and to catalyze the synthesis of glycerophospholipid precursors from arachidonyl-CoA and lysophosphatidylcholine. The protein may function in membrane biogenesis and production of platelet-activating factor in inflammatory cells. The deduced full-length protein contains an N-terminal catalytic domain and 2 cation-binding EF-hand motifs in its C-terminal region.Epitope-tagged Lpcat2 was expressed mainly in the ER and Golgi of transfected Chinese hamster ovary cells.

Alternative Names: LPCAT2; AYTL1; DKFZp686H22112; FLJ20481; LysoPAFAT; acetyl-CoA:lyso-PAF acetyltransferase; acyl-CoA:lysophosphatidylcholine acyltransferase 2; acyltransferase like 1; lyso-platelet-activating factor (PAF) acet

Search name: LPCAT2; AYTL1; DKFZp686H22112; FLJ20481; LysoPAFAT; acetyl-CoA:lyso-PAF acetyltransferase; acyl-CoA:lysophosphatidylcholine acyltransferase 2; acyltransferase like 1; lyso-platelet-activating factor (PAF) acet

Tag: LPCAT2