Mouse MBP (Myelin Basic Protein) ELISA Kit | ELK3280

Mouse MBP (Myelin Basic Protein) ELISA Kit | ELK3280 | Gentaur UK, US & Europe Distribution

Alternative Names: PRG2; BMPG; Proteoglycan 2; Bone Marrow, Natural Killer Cell Activator; Eosinophil Granule Major Basic Protein; Proteoglycan 2, Bone Marrow; Pregnancy-associated major basic

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.114 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Infection immunity;Rheumatology;Dermatology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myelin Basic Protein (MBP) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Myelin Basic Protein (MBP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myelin Basic Protein (MBP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myelin Basic Protein (MBP) in the samples is then determined by comparing the OD of the samples to the standard curve.