Mouse Myelin basic protein (MBP) ELISA Kit | KTE71103

Mouse Myelin basic protein (MBP) ELISA Kit | KTE71103 | Gentaur UK, US & Europe Distribution

Application: This Mouse Myelin basic protein (MBP) ELISA Kit employs a two-site sandwich ELISA to quantitate MBP in samples. An antibody specific for MBP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMBP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MBP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MBP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Myelin basic protein microplate
• Mouse Myelin basic protein standard
• Mouse Myelin basic protein detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Myelin basic protein (MBP) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MBP. No significant cross-reactivity or interference between Mouse MBP and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Myelin basic protein (MBP) is a protein believed to be important in the process of myelination of nerves in the central nervous system (CNS) . Myelin Basic Protein (MBP) is the major constituent of the CNS myelin synthesized by oligodendrocytes and Schwann cells. MBP, PLP (proteolipid proteins) and MOG (myelin oligodendrocyte glycoprotein) are potential primary encephalitogenic target antigens in multiple sclerosis.MBP was initially sequenced in 1979 after isolation from myelin membranes. Since that time, knockout mice deficient in MBP have been developed which showed decreased amounts of CNS myelination and a progressive disorder characterized by tremors, seizures, and early death. The gene for MBP is on chromosome 18; the protein localizes to the CNS and to various cells of the hematopoietic system.

Alternative Names: MBP; MGC99675; Golli-mbp; OTTHUMP00000174383

Search name: MBP; MGC99675; Golli-mbp; OTTHUMP00000174383

Tag: MBP