Mouse Mediator of RNA polymerase II transcription subunit 17 (MED17) ELISA Kit | KTE71069

Mouse Mediator of RNA polymerase II transcription subunit 17 (MED17) ELISA Kit | KTE71069 | Gentaur UK, US & Europe Distribution

Application: This Mouse Mediator of RNA polymerase II transcription subunit 17 (MED17) ELISA Kit employs a two-site sandwich ELISA to quantitate MED17 in samples. An antibody specific for MED17 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMED17 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MED17 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MED17 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Mediator of RNA polymerase II transcription subunit 17 microplate
• Mouse Mediator of RNA polymerase II transcription subunit 17 standard
• Mouse Mediator of RNA polymerase II transcription subunit 17 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Mediator of RNA polymerase II transcription subunit 17 (MED17) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MED17. No significant cross-reactivity or interference between Mouse MED17 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by MED17 is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor- (TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.

Alternative Names: MED17; CRSP6; CRSP77; DRIP80; FLJ10812; TRAP80; cofactor required for Sp1 transcriptional activation; subunit 6 (77kD) ; cofactor required for Sp1 transcriptional activation; subunit 6; 77kDa; thyroid hormone

Search name: MED17; CRSP6; CRSP77; DRIP80; FLJ10812; TRAP80; cofactor required for Sp1 transcriptional activation; subunit 6 (77kD) ; cofactor required for Sp1 transcriptional activation; subunit 6; 77kDa; thyroid hormone

Tag: MED17