Mouse Mediator of RNA polymerase II transcription subunit 19 (MED19) ELISA Kit | KTE71067

Mouse Mediator of RNA polymerase II transcription subunit 19 (MED19) ELISA Kit | KTE71067 | Gentaur UK, US & Europe Distribution

Application: This Mouse Mediator of RNA polymerase II transcription subunit 19 (MED19) ELISA Kit employs a two-site sandwich ELISA to quantitate MED19 in samples. An antibody specific for MED19 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMED19 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MED19 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MED19 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Mediator of RNA polymerase II transcription subunit 19 microplate
• Mouse Mediator of RNA polymerase II transcription subunit 19 standard
• Mouse Mediator of RNA polymerase II transcription subunit 19 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Mediator of RNA polymerase II transcription subunit 19 (MED19) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MED19. No significant cross-reactivity or interference between Mouse MED19 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: The protein encoded by MED19 is a subunit of the Mediator complex, which binds to gene-specific regulatory factors and provides support for the basal RNA polymerase II transcription machinery. MED19 has been implicated in the growth of several types of cancer, and inhibition of its expression inhibits the growth and spread of these cancers. Two transcript variants encoding different isoforms have been found for MED19.

Alternative Names: MED19; DT2P1G7; LCMR1; lung cancer metastasis-related protein 1; mediator of RNA polymerase transcription; subunit 19 homolog

Search name: MED19; DT2P1G7; LCMR1; lung cancer metastasis-related protein 1; mediator of RNA polymerase transcription; subunit 19 homolog

Tag: MED19