Mouse Melanocortin-2 receptor accessory protein (MRAP) ELISA Kit | KTE70957

Mouse Melanocortin-2 receptor accessory protein (MRAP) ELISA Kit | KTE70957 | Gentaur UK, US & Europe Distribution

Application: This Mouse Melanocortin-2 receptor accessory protein (MRAP) ELISA Kit employs a two-site sandwich ELISA to quantitate MRAP in samples. An antibody specific for MRAP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMRAP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MRAP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MRAP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Melanocortin-2 receptor accessory protein microplate
• Mouse Melanocortin-2 receptor accessory protein standard
• Mouse Melanocortin-2 receptor accessory protein detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Melanocortin-2 receptor accessory protein (MRAP) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MRAP. No significant cross-reactivity or interference between Mouse MRAP and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: MRAP encodes a melanocortin receptor-interacting protein. The melanocortin 2 receptor accessory protein regulates trafficking and function of the melanocortin 2 receptor in the adrenal gland. The encoded protein can also modulate signaling of other melanocortin receptors. Mutations in this gene have been associated with familial glucocorticoid deficiency type 2. Alternatively spliced transcript variants have been described.

Alternative Names: MRAP; B27; C21orf61; FALP; FGD2; GCCD2; fat cell-specific low molecular weight protein

Search name: MRAP; B27; C21orf61; FALP; FGD2; GCCD2; fat cell-specific low molecular weight protein

Tag: MRAP