(Mouse) Melk Antibody (C-term) [AMM08723G]

(Mouse) Melk Antibody (C-term) [AMM08723G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: M

Specificity: This mouse Melk antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 434-468 amino acids from the C-terminal region of mouse Melk.

Cellular Localisation: Cell membrane; Peripheral membrane protein

Molecular Weight: 72729

Clone: Polyclonal

Gene Name: Melk

Gene ID: 17279

Function: Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis.

Summary: Tissue Location: Expressed in testis, ovary, thymus, spleen and T- cell. Expressed by neural progenitors: highly enriched in cultures containing multipotent progenitors.

Form: N/A

Storage: Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Application: WB, FC

Dilution: FC--1:25 WB--1:1000

Synonyms: Maternal embryonic leucine zipper kinase, Protein kinase PK38, mPK38, Tyrosine-protein kinase MELK, Melk, Kiaa0175, Pk38