Mouse Midkine (MDK) ELISA Kit | KTE71083

Mouse Midkine (MDK) ELISA Kit | KTE71083 | Gentaur UK, US & Europe Distribution

Application: This Mouse Midkine (MDK) ELISA Kit employs a two-site sandwich ELISA to quantitate MDK in samples. An antibody specific for MDK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMDK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MDK is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MDK bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Midkine microplate
• Mouse Midkine standard
• Mouse Midkine detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Midkine (MDK) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MDK. No significant cross-reactivity or interference between Mouse MDK and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Mdk encodes a secreted growth factor that belongs to the pleiotrophin/midkine heparin-binding protein family and functions in a variety of biological processes. The encoded cytokine promotes the growth, differentiation, survival and migration of several target cells including leucocytes involved in inflammation. This protein plays a role in the formation of scar tissue and intraperitoneal adhesions, and promotes neurite outgrowth and neuron survival. The protein encoded by Mdk is associated with obesity and inhibition of insulin signaling in fat cells. A pseudogene of Mdk is present on chromosome 11. Alternative splicing results in multiple transcript variants.

Alternative Names: MDK; FLJ27379; MK; NEGF2; OTTHUMP00000197041; OTTHUMP00000197042; OTTHUMP00000197045; midkine

Search name: MDK; FLJ27379; MK; NEGF2; OTTHUMP00000197041; OTTHUMP00000197042; OTTHUMP00000197045; midkine

Tag: MDK