Mouse Mitochondrial import receptor subunit TOM34 (TOMM34) ELISA Kit | KTE70074

Mouse Mitochondrial import receptor subunit TOM34 (TOMM34) ELISA Kit | KTE70074 | Gentaur UK, US & Europe Distribution

Application: This Mouse Mitochondrial import receptor subunit TOM34 (TOMM34) ELISA Kit employs a two-site sandwich ELISA to quantitate TOMM34 in samples. An antibody specific for TOMM34 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTOMM34 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TOMM34 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TOMM34 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Mitochondrial import receptor subunit TOM34 microplate
• Mouse Mitochondrial import receptor subunit TOM34 standard
• Mouse Mitochondrial import receptor subunit TOM34 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Mitochondrial import receptor subunit TOM34 (TOMM34) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TOMM34. No significant cross-reactivity or interference between Mouse TOMM34 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: TOMM34 is involved in the import of precursor proteins into mitochondria. The encoded protein has a chaperone-like activity, binding the mature portion of unfolded proteins and aiding their import into mitochondria. This protein, which is found in the cytoplasm and sometimes associated with the outer mitochondrial membrane, has a weak ATPase activity and contains 6 TPR repeats. hTom34 is displayed on the surface of mitochondria and is resistant to extraction under alkaline conditions. Antibodies raised against hTom34 specifically inhibit in vitro import of the mitochondrial precursor protein preornithine transcarbamylase into mitochondria isolated from rat liver. Based on trypsin digestion experiments, the receptor has a large (27 kDa) C-terminal domain exposed to the cytosol.

Alternative Names: TOMM34; HTOM34P; TOM34; URCC3; mitochondrial import receptor subunit TOM34; outer mitochondrial membrane translocase (34kD)

Search name: TOMM34; HTOM34P; TOM34; URCC3; mitochondrial import receptor subunit TOM34; outer mitochondrial membrane translocase (34kD)

Tag: TOMM34