855

Mouse MMP15 (Matrix Metalloproteinase 15) ELISA Kit | ELK2903

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SKU:
855-ELK2903-GEN
$2,058.00 - $2,464.00

Description

Mouse MMP15 (Matrix Metalloproteinase 15) ELISA Kit | ELK2903 | Gentaur UK, US & Europe Distribution

Alternative Names: SMCP-2; MTMMP2; MT2-MMP; Membrane-Inserted; Membrane-type matrix metalloproteinase 2; Membrane-type-2 matrix metalloproteinase

Species: Mouse

Assay Type: Sandwich

Sensitivity: 31 pg/mL

Standard: 5000 pg/mL

Detection Range: 78.13-5000 pg/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase;Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 15 (MMP15) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Matrix Metalloproteinase 15 (MMP15) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Matrix Metalloproteinase 15 (MMP15), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Matrix Metalloproteinase 15 (MMP15) in the samples is then determined by comparing the OD of the samples to the standard curve.

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