Mouse Myc-associated zinc finger protein (MAZ) ELISA Kit | KTE71107

Mouse Myc-associated zinc finger protein (MAZ) ELISA Kit | KTE71107 | Gentaur UK, US & Europe Distribution

Application: This Mouse Myc-associated zinc finger protein (MAZ) ELISA Kit employs a two-site sandwich ELISA to quantitate MAZ in samples. An antibody specific for MAZ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAZ present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAZ is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAZ bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Myc-associated zinc finger protein microplate
• Mouse Myc-associated zinc finger protein standard
• Mouse Myc-associated zinc finger protein detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Myc-associated zinc finger protein (MAZ) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MAZ. No significant cross-reactivity or interference between Mouse MAZ and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: The MYC gene product functions as a DNA-specific transcription factor. A related polypeptide called MAX, for MYC-associated factor X, dimerizes with MYC to optimize DNA binding. A paucity of negative regulatory elements have been reported for the MYC gene which regulate transcription from 2 initiation sites specified by the P1 and P2 promoters. ME1a1, a 16-bp nuclear nuclear factor binding site residing between MYC P1 and P2 transcription initiation sites, is required for P2 activity. MAZ, Its mRNA was present in all tissues tested (except kidney) as a doublet of approximately 2.5 to 2.7 kb, along with differentially expressed minor species. The authors speculated that MAZ may encode a transcription factor with dual roles in transcription initiation and termination.

Alternative Names: MAZ; PUR1; Pur-1; SAF-1; SAF-2; SAF-3; ZF87; ZNF801; Zif87; MYC-associated zinc finger protein; purine-binding transcription factor; serum amyloid A activating factor 1; serum amyloid A activating factor 2;

Search name: MAZ; PUR1; Pur-1; SAF-1; SAF-2; SAF-3; ZF87; ZNF801; Zif87; MYC-associated zinc finger protein; purine-binding transcription factor; serum amyloid A activating factor 1; serum amyloid A activating factor 2;

Tag: MAZ