Mouse Myosin-Vb (MYO5B) ELISA Kit | KTE70865

Mouse Myosin-Vb (MYO5B) ELISA Kit | KTE70865 | Gentaur UK, US & Europe Distribution

Application: This Mouse Myosin-Vb (MYO5B) ELISA Kit employs a two-site sandwich ELISA to quantitate MYO5B in samples. An antibody specific for MYO5B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYO5B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYO5B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYO5B bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Myosin-Vb microplate
• Mouse Myosin-Vb standard
• Mouse Myosin-Vb detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Myosin-Vb (MYO5B) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MYO5B. No significant cross-reactivity or interference between Mouse MYO5B and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: May be involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Required in a complex with RAB11A and RAB11FIP2 for the transport of NPC1L1 to the plasma membrane. Together with RAB11A participates in CFTR trafficking to the plasma membrane and TF (transferrin) recycling in nonpolarized cells. Together with RAB11A and RAB8A participates in epithelial cell polarization. Together with RAB25 regulates transcytosis.

Alternative Names: MYO5B; KIAA1119; MYO5B variant protein

Search name: MYO5B; KIAA1119; MYO5B variant protein

Tag: MYO5B