Description
Mouse ND1 (NADH Dehydrogenase 1) ELISA Kit | ELK8001 | Gentaur UK, US & Europe Distribution
Alternative Names: MT-ND1; MTND1; NAD1; NADH-ubiquinone oxidoreductase chain 1; Mitochondrially Encoded NADH Dehydrogenase 1
Species: Mouse
Assay Type: Sandwich
Sensitivity: 0.115 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: tissue homogenates, cell lysates and other biological fluids
Assay Lenght: 3.5h
Research Area: Enzyme & Kinase
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to NADH Dehydrogenase 1 (ND1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to NADH Dehydrogenase 1 (ND1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NADH Dehydrogenase 1 (ND1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NADH Dehydrogenase 1 (ND1) in the samples is then determined by comparing the OD of the samples to the standard curve.