Mouse NFKB-p65 (Nuclear Factor Kappa B p65) ELISA Kit | ELK9154

Mouse NFKB-p65 (Nuclear Factor Kappa B p65) ELISA Kit | ELK9154 | Gentaur UK, US & Europe Distribution

Alternative Names: NF kappa B p65delta3; NFKB3

Species: Mouse

Assay Type: Sandwich

Sensitivity: 12.6 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: serum, plasma, tissue homogenates

Assay Lenght: 3.5h

Research Area: Immunology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclear Factor Kappa B p65 (NFKB-p65) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Nuclear Factor Kappa B p65 (NFKB-p65) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nuclear Factor Kappa B p65 (NFKB-p65), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nuclear Factor Kappa B p65 (NFKB-p65) in the samples is then determined by comparing the OD of the samples to the standard curve.