Mouse Nlrp6 Antibody (Center) [APR04126G]

Mouse Nlrp6 Antibody (Center) [APR04126G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: M

Specificity: This Mouse Nlrp6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 343-372 amino acids from the Central region of mouse Nlrp6.

Cellular Localisation: [Isoform 1]: Cytoplasm {ECO:0000250|UniProtKB:Q63035}. Inflammasome {ECO:0000250|UniProtKB:P59044}. Cell membrane {ECO:0000250|UniProtKB:Q63035}. Nucleus membrane {ECO:0000250|UniProtKB:Q63035}

Molecular Weight: 97402

Clone: Polyclonal

Gene Name: Nlrp6

Gene ID: 101613

Function: As the sensor component of the NLRP6 inflammasome, plays a crucial role in innate immunity and inflammation. In response to yet unidentified signals, initiates the formation of the inflammasome polymeric complex, made of NLRP6, PYCARD and CASP1 (or possibly CASP4/CASP11) . Recruitment of proCASP1 to the inflammasome promotes its activation and CASP1-catalyzed IL1B and IL18 maturation and secretion in the extracellular milieu. The precise NLRP6 activation stimulus has not been identified yet (By similarity) (PubMed:21593405) . Essential for gut mucosal self-renewal and proliferation (PubMed:21593405, PubMed:21565393, PubMed:21543645) . Maintains intestinal homeostasis and a healthy intestinal microbiota (PubMed:21565393, PubMed:22763455) . This function is, at least partially, mediated by IL18, and not IL1B, produced by nonhematopoietic cells (PubMed:21565393) . Influences intestinal barrier function and microbial homeostasis through the regulation of goblet cell mucus secretion. Acts by promoting autophagy in goblet cells, an essential step for mucus granule exocytosis. Its role in goblet cell physiology is inflammasome-dependent, but IL1B- and IL18-independent (PubMed:24581500) . During systemic bacterial infections, may negatively regulate inflammatory signaling and inhibit the influx of monocytes and neutrophils to the circulation and to the peritoneum (PubMed:22763455) . May promote peripheral nerve recovery following injury via an inflammasome-independent mechanism (PubMed:26253422) .

Summary: Tissue Location: Highly expressed in the gastrointestinal tract, predominantly in colonic myofibroblasts and in colonic epithelial and endothelial cells. Within the intestinal mucosa, highly expressed by goblet cells. Also expressed in hepatocytes and in immune cells, including CD4 (+) and CD8 (+) T-cells, dendritic cells, mastocytes and peritoneal macrophages, as well as in lung, kidney, bladder and gonads

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: IHC-P-Leica, WB

Dilution: IHC-P-Leica--1:500 WB--1:500 WB--1:1000 WB--1:2000

Synonyms: NACHT, LRR and PYD domains-containing protein 6, Angiotensin II/vasopressin receptor, PYRIN-containing APAF1-like protein 5-like, Nlrp6, Nalp6, Pypaf5