Mouse NOG (Noggin) ELISA Kit | ELK7666

Mouse NOG (Noggin) ELISA Kit | ELK7666 | Gentaur UK, US & Europe Distribution

Alternative Names: SYM1; SYNS1; Synostoses (Multiple) Syndrome 1; Symphalangism 1 (Proximal)

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.066 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction;Immune molecule;Neuro science;Bone metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Noggin (NOG) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Noggin (NOG) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Noggin (NOG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Noggin (NOG) in the samples is then determined by comparing the OD of the samples to the standard curve.