Mouse NT-ProBNP (N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit | ELK5445

Mouse NT-ProBNP (N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit | ELK5445 | Gentaur UK, US & Europe Distribution

Alternative Names: NT-Pro-BNP; ; N-BNP; ANFB_HUMAN; BNP (1-32) ; BNP (5-29) ; BNP-32; Gamma-brain natriuretic peptide; nppb

Species: Mouse

Assay Type: Sandwich

Sensitivity: 28 pg/mL

Standard: 5000 pg/mL

Detection Range: 78.13-5000 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Endocrinology;Cardiovascular biology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) in the samples is then determined by comparing the OD of the samples to the standard curve.