749

Mouse Nuclear receptor subfamily 5 group A member 2 (NR5A2) ELISA Kit | KTE71380

(No reviews yet) Write a Review
SKU:
749-KTE71380
Availability:
Usually ships in 5 working days
NULL627.00 - NULL1,032.00

Description

Mouse Nuclear receptor subfamily 5 group A member 2 (NR5A2) ELISA Kit | KTE71380 | Gentaur UK, US & Europe Distribution

Application: This Mouse Nuclear receptor subfamily 5 group A member 2 (NR5A2) ELISA Kit employs a two-site sandwich ELISA to quantitate NR5A2 in samples. An antibody specific for NR5A2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNR5A2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NR5A2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NR5A2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Nuclear receptor subfamily 5 group A member 2 microplate
• Mouse Nuclear receptor subfamily 5 group A member 2 standard
• Mouse Nuclear receptor subfamily 5 group A member 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Nuclear receptor subfamily 5 group A member 2 (NR5A2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse NR5A2. No significant cross-reactivity or interference between Mouse NR5A2 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: By means of yeast 1-hybrid screening of a liver cDNA library, Li et al. (1998) cloned a cDNA encoding a novel hepatocyte transcription factor, which they called HB1F for human B1-binding factor. The deduced 495-amino acid protein, which has a molecular mass of 54 kD, belongs to the fushi tarazu factor-1 (FTZ-F1) subfamily of orphan nuclear receptors and is closely related to steroidogenic factor-1 (SF1), another member of this subfamily. HB1F contains a DNA-binding domain with 2 zinc finger motifs, an FTZ-F1 box, and a ligand-binding domain. Northern blot analysis revealed that HB1F is expressed in liver, pancreas, and lung as a 5.2-kb transcript. An additional transcript of 3.8 kb was present in hepatoma cells HepG2. The authors identified 2 HB1F isoforms which differ in their A/B region.

Alternative Names: NR5A2; B1F; B1F2; CPF; FTF; FTZ-F1; FTZ-F1beta; LRH-1; LRH1; hB1F; hB1F-2; CYP7A promoter-binding factor; b1-binding factor; hepatocyte transcription factor which activates enhancer of hepatitis B virus;

Search name: NR5A2; B1F; B1F2; CPF; FTF; FTZ-F1; FTZ-F1beta; LRH-1; LRH1; hB1F; hB1F-2; CYP7A promoter-binding factor; b1-binding factor; hepatocyte transcription factor which activates enhancer of hepatitis B virus;

Tag: NR5A2

View AllClose