Mouse P-selectin glycoprotein ligand 1 (SELPLG) ELISA Kit | AE20196MO

Mouse P-selectin glycoprotein ligand 1 (SELPLG) ELISA Kit | AE20196MO | Gentaur UK, US & Europe Distribution

Species Reactivity: Mouse (Mus musculus)

Abbreviation: SELPLG

Alternative Name: CD162; CLA; PSGL-1; PSGL1; cutaneous lymphocyte-associated associated antigen

Application: ELISA

Range: 0.156-10 ng/mL

Sensitivity: 0.066 ng/mL

Intra-Assay: ≤4.7%

Inter-Assay: ≤9.8%

Recovery: 1, 03

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate SELPLG in samples. An antibody specific for SELPLG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySELPLG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SELPLG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SELPLG bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: SELPLG is the high affinity counter-receptor for P-selectin on myeloid cells and stimulated T lymphocytes. As such, it plays a critical role in the tethering of these cells to activated platelets or endothelia expressing P-selectin. The organization of the SELPG gene closely resembles that of CD43 and the human platelet glycoprotein GpIb-alpha both of which have an intron in the 5-prime-noncoding region, a long second exon containing the complete coding region, and TATA-less promoters. P-selectin glycoprotein ligand-1 is a glycoprotein found on white blood cells and endothelial cells that binds to P-selectin (P stands for platelet) . It is one of a family of selectins that includes E-selectin (endothelial) and L-selectin (leukocyte) . PSGL-1 can bind to all three members of the family but binds best (with the highest affinity) to P-selectin.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .