Description
Mouse PDK4 (Pyruvate Dehydrogenase Kinase Isozyme 4) ELISA Kit | ELK5009 | Gentaur UK, US & Europe Distribution
Alternative Names: [Pyruvate dehydrogenase (acetyl-transferring) ] kinase isozyme 4, mitochondrial
Species: Mouse
Assay Type: Sandwich
Sensitivity: 0.127 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Metabolic pathway;Cardiovascular biology
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pyruvate Dehydrogenase Kinase Isozyme 4 (PDK4) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pyruvate Dehydrogenase Kinase Isozyme 4 (PDK4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pyruvate Dehydrogenase Kinase Isozyme 4 (PDK4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pyruvate Dehydrogenase Kinase Isozyme 4 (PDK4) in the samples is then determined by comparing the OD of the samples to the standard curve.