Mouse Peroxisomal N (PAOX) ELISA Kit | AE28699MO

Mouse Peroxisomal N (PAOX) ELISA Kit | AE28699MO | Gentaur UK, US & Europe Distribution

Species Reactivity: Mouse (Mus musculus)

Abbreviation: PAOX

Alternative Name: RP11-122K13.11; DKFZp434J245; MGC45464; PAO; peroxisomal N1-acetyl-spermine/spermidine oxidase|polyamine oxidase

Application: ELISA

Range: 78.12-5000 pg/mL

Sensitivity: 32 pg/mL

Intra-Assay: ≤4.5%

Inter-Assay: ≤8.1%

Recovery: 0, 89

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate PAOX in samples. An antibody specific for PAOX has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPAOX present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PAOX is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PAOX bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Polyamine Oxidaseis an enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-NH group of donors with oxygen as acceptor. Widely expressed. Not detected in spleen. Expressed at lower level in neoplastic tissues. Flavoenzyme which catalyzes the oxidation of N (1) -acetylspermine to spermidine and is thus involved in the polyamine back-conversion. Can also oxidize N (1) -acetylspermidine to putrescine. Substrate specificity: N (1) -acetylspermine = N (1) -acetylspermidine > N1, N (12) -diacylspermine >> spermine. Does not oxidize spermidine. Plays an important role in the regulation of polyamine intracellular concentration and has the potential to act as a determinant of cellular sensitivity to the antitumor polyamine analogs.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .