Mouse Peroxisomal N (PAOX) ELISA Kit | KTE70791

Mouse Peroxisomal N (PAOX) ELISA Kit | KTE70791 | Gentaur UK, US & Europe Distribution

Application: This Mouse Peroxisomal N (PAOX) ELISA Kit employs a two-site sandwich ELISA to quantitate PAOX in samples. An antibody specific for PAOX has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPAOX present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PAOX is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PAOX bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Peroxisomal N microplate
• Mouse Peroxisomal N standard
• Mouse Peroxisomal N detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Peroxisomal N (PAOX) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PAOX. No significant cross-reactivity or interference between Mouse PAOX and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Polyamine Oxidaseis an enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-NH group of donors with oxygen as acceptor. Widely expressed. Not detected in spleen. Expressed at lower level in neoplastic tissues. Flavoenzyme which catalyzes the oxidation of N (1) -acetylspermine to spermidine and is thus involved in the polyamine back-conversion. Can also oxidize N (1) -acetylspermidine to putrescine. Substrate specificity: N (1) -acetylspermine = N (1) -acetylspermidine > N1, N (12) -diacylspermine >> spermine. Does not oxidize spermidine. Plays an important role in the regulation of polyamine intracellular concentration and has the potential to act as a determinant of cellular sensitivity to the antitumor polyamine analogs.

Alternative Names: PAOX; RP11-122K13.11; DKFZp434J245; MGC45464; PAO; peroxisomal N1-acetyl-spermine/spermidine oxidase; polyamine oxidase

Search name: PAOX; RP11-122K13.11; DKFZp434J245; MGC45464; PAO; peroxisomal N1-acetyl-spermine/spermidine oxidase; polyamine oxidase

Tag: PAOX