Mouse PGE2 (Prostaglandin E2) ELISA Kit | ELK8699

Mouse PGE2 (Prostaglandin E2) ELISA Kit | ELK8699 | Gentaur UK, US & Europe Distribution

Alternative Names: PG-E2

Species: Mouse

Assay Type: Competitive Inhibition

Sensitivity: 0.97 pg/mL

Standard: 200 pg/mL

Detection Range: 3.13-200 pg/mL

Sample Type: serum, plasma and other biological fluids

Assay Lenght: 2h

Research Area: Metabolic pathway;Infection immunity;Endocrinology;Hormone metabolism

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Prostaglandin E2 (PGE2) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Prostaglandin E2 (PGE2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Prostaglandin E2 (PGE2) in the samples is then determined by comparing the OD of the samples to the standard curve.