Mouse Phenylalanine-4-hydroxylase (PAH) ELISA Kit | KTE71459

Mouse Phenylalanine-4-hydroxylase (PAH) ELISA Kit | KTE71459 | Gentaur UK, US & Europe Distribution

Application: This Mouse Phenylalanine-4-hydroxylase (PAH) ELISA Kit employs a two-site sandwich ELISA to quantitate PAH in samples. An antibody specific for PAH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPAH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PAH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PAH bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Phenylalanine-4-hydroxylase microplate
• Mouse Phenylalanine-4-hydroxylase standard
• Mouse Phenylalanine-4-hydroxylase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Phenylalanine-4-hydroxylase (PAH) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PAH. No significant cross-reactivity or interference between Mouse PAH and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Phenylalanine hydroxylase is the rate-limiting enzyme of the metabolic pathway which degrades excess phenylalanine. The other substrates in the reaction are molecular oxygen and tetrahydrobiopterin. Tetrahydrobiopterin is a member of the group of redox biochemicals known as pteridines. PAH is the gene that encodes for phenylalanine hydroxylase. It was the research on phenylalanine hydroxylase by Seymour Kaufman that led to the discovery of tetrahydrobiopterin as a biological cofactor. Phenylalanine hydroxylase is a tetramer composed of four monomers, that is, composed of 4 identical subunits. Each subunit is in turn composed of three domains, a regulatory domain, a catalytic domain, and a tetramerization domain

Alternative Names: PAH; PH; PKU; PKU1;

Search name: PAH; PH; PKU; PKU1;

Tag: PAH