Mouse Phosphoinositide 3-kinase adapter protein 1 (PIK3AP1) ELISA Kit | KTE70754

Mouse Phosphoinositide 3-kinase adapter protein 1 (PIK3AP1) ELISA Kit | KTE70754 | Gentaur UK, US & Europe Distribution

Application: This Mouse Phosphoinositide 3-kinase adapter protein 1 (PIK3AP1) ELISA Kit employs a two-site sandwich ELISA to quantitate PIK3AP1 in samples. An antibody specific for PIK3AP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPIK3AP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIK3AP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIK3AP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Phosphoinositide 3-kinase adapter protein 1 microplate
• Mouse Phosphoinositide 3-kinase adapter protein 1 standard
• Mouse Phosphoinositide 3-kinase adapter protein 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Phosphoinositide 3-kinase adapter protein 1 (PIK3AP1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PIK3AP1. No significant cross-reactivity or interference between Mouse PIK3AP1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Using an affinity purification strategy with the N-terminal SH2 domain of the phosphoinositide 3-kinase (PI3K) p85 subunit and anti-phosphotyrosine antibody, followed by screening a chicken DT40 B-cell cDNA library and a mouse spleen cDNA library, Okada et al. (2000) isolated cDNAs encoding chicken and mouse Pik3ap1, which they called Bcap. They also identified Bcap splice variants. Northern blot analysis of mouse tissues detected expression predominantly in spleen, with lower levels in thymus, liver, and lung. Expression was also detected in mouse macrophage and B-cell lines, but not in T-cell, plasma cell, or mast cell lines.Using mouse and chicken B- cell lines, Okada et al. (2000) determined that Bcap is a protein tyrosine kinase substrate that couples B-cell receptor to PI3K activation.

Alternative Names: PIK3AP1; BCAP; RP11-34E5.3; 1810044J04Rik; B cell adaptor protein

Search name: PIK3AP1; BCAP; RP11-34E5.3; 1810044J04Rik; B cell adaptor protein

Tag: PIK3AP1