Mouse PIICP (Procollagen II C-Terminal Propeptide) ELISA Kit | ELK5532

Mouse PIICP (Procollagen II C-Terminal Propeptide) ELISA Kit | ELK5532 | Gentaur UK, US & Europe Distribution

Alternative Names: P2CP; C-Propeptide Of Type II Procollagen; Procollagen II Carboxy Terminal Propeptide

Species: Mouse

Assay Type: Competitive Inhibition

Sensitivity: 1.49 ng/mL

Standard: 300 ng/mL

Detection Range: 4.69-300 ng/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 2h

Research Area: Infection immunity;Bone metabolism;Rheumatology

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Procollagen II C-Terminal Propeptide (PIICP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Procollagen II C-Terminal Propeptide (PIICP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Procollagen II C-Terminal Propeptide (PIICP) in the samples is then determined by comparing the OD of the samples to the standard curve.