Mouse Pituitary homeobox 1 (PITX1) ELISA Kit | KTE70746

Mouse Pituitary homeobox 1 (PITX1) ELISA Kit | KTE70746 | Gentaur UK, US & Europe Distribution

Application: This Mouse Pituitary homeobox 1 (PITX1) ELISA Kit employs a two-site sandwich ELISA to quantitate PITX1 in samples. An antibody specific for PITX1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPITX1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PITX1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PITX1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Pituitary homeobox 1 microplate
• Mouse Pituitary homeobox 1 standard
• Mouse Pituitary homeobox 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Pituitary homeobox 1 (PITX1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PITX1. No significant cross-reactivity or interference between Mouse PITX1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: PITX1 encodes a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. According to an October 2008 press release by the Washington University School of Medicine, the gene is the first gene thought to be involved with clubfoot. Members of this family are involved in organ development and left-right asymmetry. This protein acts as a transcriptional regulator involved in basal and hormone-regulated activity of prolactin and it could be associated with autism. The putative encoded mouse and human proteins share 100% identity in the homeodomain and are 88% and 97% conserved in the N- and C-terminal regions, respectively. Intron/exon boundaries are also conserved.

Alternative Names: PITX1; BFT; CCF; POTX; PTX1; hindlimb expressed homeobox protein backfoot; paired-like homeodomain transcription factor 1; pituitary homeo box 1; pituitary otx-related factor

Search name: PITX1; BFT; CCF; POTX; PTX1; hindlimb expressed homeobox protein backfoot; paired-like homeodomain transcription factor 1; pituitary homeo box 1; pituitary otx-related factor

Tag: PITX1