Mouse PRKG1 (Protein Kinase, cGMP Dependent Type I) ELISA Kit | ELK7207

Mouse PRKG1 (Protein Kinase, cGMP Dependent Type I) ELISA Kit | ELK7207 | Gentaur UK, US & Europe Distribution

Alternative Names: CGKI; PGK; PRKG1B; PRKGR1B; cGKI-BETA; cGKI-alpha; cGMP-dependent protein kinase 1

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.059 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase, cGMP Dependent Type I (PRKG1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Protein Kinase, cGMP Dependent Type I (PRKG1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase, cGMP Dependent Type I (PRKG1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Kinase, cGMP Dependent Type I (PRKG1) in the samples is then determined by comparing the OD of the samples to the standard curve.