Mouse PRNP (Prion Protein) ELISA Kit | ELK5103

Mouse PRNP (Prion Protein) ELISA Kit | ELK5103 | Gentaur UK, US & Europe Distribution

Alternative Names: CD230; GSS; ASCR; CJD; PRIP; PrP; PrP2730; PrPc; Major prion protein; Creutzfeldt-Jakob Disease; Gerstmann-Strausler-Scheinker Syndrome; Fatal Familial Insomnia

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.058 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction;CD & Adhesion molecule;Infection immunity;Neuro science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prion Protein (PRNP) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Prion Protein (PRNP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prion Protein (PRNP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Prion Protein (PRNP) in the samples is then determined by comparing the OD of the samples to the standard curve.