Mouse Protein phosphatase 1 regulatory subunit 1B (PPP1R1B) ELISA Kit | KTE70701

Mouse Protein phosphatase 1 regulatory subunit 1B (PPP1R1B) ELISA Kit | KTE70701 | Gentaur UK, US & Europe Distribution

Application: This Mouse Protein phosphatase 1 regulatory subunit 1B (PPP1R1B) ELISA Kit employs a two-site sandwich ELISA to quantitate PPP1R1B in samples. An antibody specific for PPP1R1B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPPP1R1B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPP1R1B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPP1R1B bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Protein phosphatase 1 regulatory subunit 1B microplate
• Mouse Protein phosphatase 1 regulatory subunit 1B standard
• Mouse Protein phosphatase 1 regulatory subunit 1B detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Protein phosphatase 1 regulatory subunit 1B (PPP1R1B) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PPP1R1B. No significant cross-reactivity or interference between Mouse PPP1R1B and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Midbrain dopaminergic neurons play a critical role in multiple brain functions, and abnormal signaling through dopaminergic pathways has been implicated in several major neurologic and psychiatric disorders.In the densely dopamine- and glutamate-innervated rat caudate-putamen, DARPP32 is expressed in medium-sized spiny neurons that also express dopamine D1 receptors. The function of DARPP32 seems to be regulated by receptor stimulation. Both dopaminergic and glutamatergic (NMDA) receptor stimulation regulate the extent of DARPP32 phosphorylation, but in opposite directions. Dopamine D1 receptor stimulation enhances cAMP formation, resulting in the phosphorylation of DARPP32; phosphorylated DARPP32 is a potent protein phosphatase-1.

Alternative Names: PPP1R1B; DARPP-32; DARPP32; FLJ20940; OTTHUMP00000164276; dopamine and cAMP regulated phosphoprotein; dopamine and cAMP-regulated neuronal phosphoprotein 32; protein phosphatase 1; regulatory (inhibitor) subuni

Search name: PPP1R1B; DARPP-32; DARPP32; FLJ20940; OTTHUMP00000164276; dopamine and cAMP regulated phosphoprotein; dopamine and cAMP-regulated neuronal phosphoprotein 32; protein phosphatase 1; regulatory (inhibitor) subuni

Tag: PPP1R1B