Mouse Rad50 Antibody (C-term) [APR31796G]

Mouse Rad50 Antibody (C-term) [APR31796G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: M

Specificity: This Mouse Rad50 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 911-938 amino acids from the C-terminal region of mouse Rad50.

Cellular Localisation: Nucleus {ECO:0000250|UniProtKB:Q92878}. Chromosome, telomere {ECO:0000250|UniProtKB:Q92878}. Chromosome {ECO:0000250|UniProtKB:Q92878}. Note=Localizes to discrete nuclear foci after treatment with genotoxic agents. {ECO:0000250|UniProtKB:Q92878}

Molecular Weight: 153488

Clone: Polyclonal

Gene Name: Rad50

Gene ID: N/A

Function: Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11 to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation (By similarity) .

Summary: Tissue Location: In adult, it is expressed at very low level in most tissues, except in heart, lung and aorta. Expressed at high level in testis.

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB

Dilution: WB--1:1000

Synonyms: DNA repair protein RAD50, mRad50, 36--, Rad50