Mouse Ras-related protein Rab-8A (RAB8A) ELISA Kit | KTE71429

Mouse Ras-related protein Rab-8A (RAB8A) ELISA Kit | KTE71429 | Gentaur UK, US & Europe Distribution

Application: This Mouse Ras-related protein Rab-8A (RAB8A) ELISA Kit employs a two-site sandwich ELISA to quantitate RAB8A in samples. An antibody specific for RAB8A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRAB8A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RAB8A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RAB8A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Ras-related protein Rab-8A microplate
• Mouse Ras-related protein Rab-8A standard
• Mouse Ras-related protein Rab-8A detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Ras-related protein Rab-8A (RAB8A) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse RAB8A. No significant cross-reactivity or interference between Mouse RAB8A and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: RAB8A is a member of the RAS superfamily which are small GTP/GDP-binding proteins with an average size of 200 amino acids. The RAS-related proteins of the RAB/YPT family may play a role in the transport of proteins from the endoplasmic reticulum to the Golgi and the plasma membrane. This protein shares 97%, 96%, and 51% similarity with the dog RAB8, mouse MEL, and mouse YPT1 proteins, respectively and contains the 4 GTP/GDP-binding sites that are present in all the RAS proteins. The putative effector-binding site of this protein is similar to that of the RAB/YPT proteins. However, this protein contains a C-terminal CAAX motif that is characteristic of many RAS superfamily members but which is not found in YPT1 and the majority of RAB proteins.

Alternative Names: RAB8A; MEL; RAB8; mel transforming oncogene; mel transforming oncogene (RAB8 homolog) ; mel transforming oncogene (derived from cell line NK14) ; mel transforming oncogene (derived from cell line NK14) - RAB8 ho

Search name: RAB8A; MEL; RAB8; mel transforming oncogene; mel transforming oncogene (RAB8 homolog) ; mel transforming oncogene (derived from cell line NK14) ; mel transforming oncogene (derived from cell line NK14) - RAB8 ho

Tag: RAB8A