Mouse Recombinant DHFR | 6383

Dihydrofolate reductase (DHFR) is a member of the reductase family of enzymes that is ubiquitously expressed in all organisms. DHFR catalyzes the NADPH-dependent reduction of dihydrofolate to tetrahydrofolate, and it is essential for the synthesis of thymidylate, purines and several amino acids. Expression of methotrexate (MTX)-resistant variants of DHFR in normal hematopoietic cells is a potential strategy to permit administration of larger doses of MTX by alleviating drug toxicity in normal cells and tissues that are drug sensitive.

6383 | Mouse Recombinant DHFR DataSheet

Biomolecule/Target: N/A

Synonyms: Mouse Recombinant DHFR

Alternates names: Dihydrofolate reductase.

Taglines: Reduces dihydrofolic acid to tetrahydrofolic acid

NCBI Gene ID #: 20302

NCBI Gene Symbol: CCL3

Gene Source: Mouse

Accession #: P10855

Recombinant: Yes

Source: E. Coli

Purity by SDS-PAGEs: 99%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Determined by its ability to chemoattract Balb/c peripheral blood MNCs and mouse Balb/c splenocytes using a concentration range of 10-100 ng/ml.

Results: >0.2 units/mg

Binding Capacity: N/A

Unit Definition: One unit will convert 1.0 µmole of 7, 8 dihydrofolate and beta-NADPH to 5, 6, 7, 8-tetrahydrofolate and beta-NADP per minute at pH 6.5 at 25°C.

Molecular Weight: 23.8 kDa (207 aa, 1-187 aa + NT His-Tag)

Concentration: 1 mg/ml

Appearance: Liquid

Physical form description: 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 0.1 M NaCl, 2 mM DTT and 10% glycerol.

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1 mg/ml. This solution can then be diluted into other aqueous buffers

Amino acid sequence: N/A