Mouse Recombinant GSTMu 1 | 6348

GSTM1 is a glutathione S-transferase that belongs to the mu class. This enzyme acts by catalyzing the reaction of glutathione with an acceptor molecule to form an S-substituted glutathione (S=sulfur). The reactions utilizing glutathione contribute the transformation of a wide variety of electrophiles, including reactive products of lipid, protein, carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress.

6348 | Mouse Recombinant GSTMu 1 DataSheet

Biomolecule/Target: N/A

Synonyms: Mouse Recombinant GSTMu 1

Alternates names: GSTM1, GST class-mu1, GST 1-1, pmGT10

Taglines: Plays a role in detoxification of electrophilic compounds

NCBI Gene ID #: 25542

NCBI Gene Symbol: CCL3

Gene Source: Mouse

Accession #: P50229

Recombinant: Yes

Source: E. Coli

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Determined by its ability to chemoattract rat peripheral macrophages using a concentration range of 50-100 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: Specific activity is defined as the amount of enzyme that conjugates 1.0 µ mole of 1-chloro-2, 4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25°C.

Molecular Weight: 28.1 kDa

Concentration: 1 mg/ml

Appearance: Liquid

Physical form description: 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, and 10% glycerol.

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1 mg/ml. This solution can then be diluted into other aqueous buffers or stored at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A