Mouse Resistin-like beta (RETNLB) ELISA Kit | AE22446MO

Mouse Resistin-like beta (RETNLB) ELISA Kit | AE22446MO | Gentaur UK, US & Europe Distribution

Species Reactivity: Mouse (Mus musculus)

Abbreviation: RETNLB

Alternative Name: FIZZ1; FIZZ2; HXCP2; RELM-beta; RELMb; RELMbeta; XCP2; C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich protein precursor 2|colon and small intestine-specific cysteine-rich protein|cy

Application: ELISA

Range: 6.25-400 pg/mL

Sensitivity: 2.54 pg/mL

Intra-Assay: ≤4.5%

Inter-Assay: ≤8.6%

Recovery: 0, 98

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate RETNLB in samples. An antibody specific for RETNLB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRETNLB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RETNLB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RETNLB bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: The 111-amino acid FIZZ2 protein, which shows 59% sequence identity to mouse Fizz2 and 47% identity to human FIZZ3, shares an N-terminal signal peptide and a C-terminal stretch of 10 cysteine residues with identical spacing with other FIZZ family members. Strong expression of mouse Fizz2 mRNA was found in the adult colon in the mucosal crypt epithelial cells. Increases in circulating resistin or RELMB levels markedly stimulated hepatic glucose production despite the presence of fixed physiologic insulin levels. This enhanced rate of glucose output was due to increased flux through glucose-6-phosphatase. The results supported the notion that a novel family of fat- and gut-derived circulating proteins modulates hepatic insulin action.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .