Mouse Ribonuclease-like protein 13 (RNASE13) ELISA Kit | KTE71427

Mouse Ribonuclease-like protein 13 (RNASE13) ELISA Kit | KTE71427 | Gentaur UK, US & Europe Distribution

Application: This Mouse Ribonuclease-like protein 13 (RNASE13) ELISA Kit employs a two-site sandwich ELISA to quantitate RNASE13 in samples. An antibody specific for RNASE13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRNASE13 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RNASE13 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RNASE13 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Ribonuclease-like protein 13 microplate
• Mouse Ribonuclease-like protein 13 standard
• Mouse Ribonuclease-like protein 13 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Ribonuclease-like protein 13 (RNASE13) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse RNASE13. No significant cross-reactivity or interference between Mouse RNASE13 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: RNASE13 belongs to the pancreatic ribonuclease family. Ribonuclease A (RNase A) is a pancreatic ribonuclease that cleaves single-stranded RNA. Bovine pancreatic RNase A is one of the classic model systems of protein science. RNase A is a relatively small protein. It can be characterized as a two-layer α + β protein that is folded in half to resemble a taco, with a deep cleft for binding the RNA substrate. The first layer is composed of three alpha helices from the N-terminal half of the protein. The second layer consist of three β-hairpins arranged in two β-sheets. The hairpins 61-74 and 105-124 form a four-stranded, antiparallel β-sheet that lies on helix 3. The longest β-hairpin 79-104 mates with a short β-strand to form a three-stranded, antiparallel β-sheet that lies on helix 2.

Alternative Names: RNASE13; Ribonuclease 13; ribonuclease; RNase A family; 13

Search name: RNASE13; Ribonuclease 13; ribonuclease; RNase A family; 13

Tag: RNASE13