Mouse RXRa (Retinoid X Receptor Alpha) ELISA Kit | ELK6828

Mouse RXRa (Retinoid X Receptor Alpha) ELISA Kit | ELK6828 | Gentaur UK, US & Europe Distribution

Alternative Names: NR2B1; RXR-A; NR2-B1; Nuclear Receptor Subfamily 2, Group B, Member 1; Retinoic acid receptor RXR-alpha

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.067 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction;Metabolic pathway;Endocrinology;Hormone metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Retinoid X Receptor Alpha (RXRa) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Retinoid X Receptor Alpha (RXRa) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Retinoid X Receptor Alpha (RXRa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Retinoid X Receptor Alpha (RXRa) in the samples is then determined by comparing the OD of the samples to the standard curve.