Mouse S1PR2 (Sphingosine 1 Phosphate Receptor 2) ELISA Kit | ELK7434

Mouse S1PR2 (Sphingosine 1 Phosphate Receptor 2) ELISA Kit | ELK7434 | Gentaur UK, US & Europe Distribution

Alternative Names: EDG5; AGR16; EDG-5; Gpcr13; H218; LPB2; S1P2; Endothelial Differentiation, Sphingolipid G-Protein-Coupled Receptor 5; Sphingosine 1-phosphate receptor Edg-5

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.114 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sphingosine 1 Phosphate Receptor 2 (S1PR2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sphingosine 1 Phosphate Receptor 2 (S1PR2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sphingosine 1 Phosphate Receptor 2 (S1PR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sphingosine 1 Phosphate Receptor 2 (S1PR2) in the samples is then determined by comparing the OD of the samples to the standard curve.