Mouse S1PR4 (Sphingosine 1 Phosphate Receptor 4) ELISA Kit | ELK7433

Mouse S1PR4 (Sphingosine 1 Phosphate Receptor 4) ELISA Kit | ELK7433 | Gentaur UK, US & Europe Distribution

Alternative Names: EDG6; LPC1; S1P4; SLP4; Endothelial Differentiation, Lysophosphatidic Acid G-Protein-Coupled Receptor 6; Sphingosine 1-phosphate receptor Edg-6

Species: Mouse

Assay Type: Sandwich

Sensitivity: 30 pg/mL

Standard: 5000 pg/mL

Detection Range: 78.13-5000 pg/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sphingosine 1 Phosphate Receptor 4 (S1PR4) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sphingosine 1 Phosphate Receptor 4 (S1PR4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sphingosine 1 Phosphate Receptor 4 (S1PR4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sphingosine 1 Phosphate Receptor 4 (S1PR4) in the samples is then determined by comparing the OD of the samples to the standard curve.