Mouse SALb (Salusin Beta) ELISA Kit | ELK5543

Mouse SALb (Salusin Beta) ELISA Kit | ELK5543 | Gentaur UK, US & Europe Distribution

Alternative Names: Salusin Beta

Species: Mouse

Assay Type: Competitive Inhibition

Sensitivity: 1.82 pg/mL

Standard: 400 pg/mL

Detection Range: 6.25-400 pg/mL

Sample Type: serum, plasma and other biological fluids

Assay Lenght: 2h

Research Area: Metabolic pathway;Endocrinology;Cardiovascular biology;Neuro science;Hormone metabolism

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Salusin Beta (SALb) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Salusin Beta (SALb) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Salusin Beta (SALb) in the samples is then determined by comparing the OD of the samples to the standard curve.