Mouse SEMA3B (Semaphorin 3B) ELISA Kit | ELK9295

Mouse SEMA3B (Semaphorin 3B) ELISA Kit | ELK9295 | Gentaur UK, US & Europe Distribution

Alternative Names: LUCA-1; SEMA5; SEMAA; SemA; semaV; Semaphorin-V; Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain 3B

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.056 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: CD & Adhesion molecule

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Semaphorin 3B (SEMA3B) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Semaphorin 3B (SEMA3B) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Semaphorin 3B (SEMA3B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Semaphorin 3B (SEMA3B) in the samples is then determined by comparing the OD of the samples to the standard curve.