Mouse Serine/threonine-protein phosphatase PP1-alpha catalytic subunit (PPP1CA) ELISA Kit | KTE70703

Mouse Serine/threonine-protein phosphatase PP1-alpha catalytic subunit (PPP1CA) ELISA Kit | KTE70703 | Gentaur UK, US & Europe Distribution

Application: This Mouse Serine/threonine-protein phosphatase PP1-alpha catalytic subunit (PPP1CA) ELISA Kit employs a two-site sandwich ELISA to quantitate PPP1CA in samples. An antibody specific for PPP1CA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPPP1CA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPP1CA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPP1CA bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Serine/threonine-protein phosphatase PP1-alpha catalytic subunit microplate
• Mouse Serine/threonine-protein phosphatase PP1-alpha catalytic subunit standard
• Mouse Serine/threonine-protein phosphatase PP1-alpha catalytic subunit detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Serine/threonine-protein phosphatase PP1-alpha catalytic subunit (PPP1CA) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PPP1CA. No significant cross-reactivity or interference between Mouse PPP1CA and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Serine/threonine-protein phosphatase PP1-alpha catalytic subunit is one of the three catalytic subunits of protein phosphatase 1 (PP1) . PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Alternative Names: PPP1CA; MGC15877; MGC1674; PP-1A; PPP1A; protein phosphatase 1; catalytic subunit; alpha; serine/threonine protein phosphatase PP1-alpha 1 catalytic subunit

Search name: PPP1CA; MGC15877; MGC1674; PP-1A; PPP1A; protein phosphatase 1; catalytic subunit; alpha; serine/threonine protein phosphatase PP1-alpha 1 catalytic subunit

Tag: PPP1CA