Mouse Solute carrier family 2, facilitated glucose transporter member 4 (SLC2A4) ELISA Kit | KTE70429

Mouse Solute carrier family 2, facilitated glucose transporter member 4 (SLC2A4) ELISA Kit | KTE70429 | Gentaur UK, US & Europe Distribution

Application: This Mouse Solute carrier family 2, facilitated glucose transporter member 4 (SLC2A4) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC2A4 in samples. An antibody specific for SLC2A4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC2A4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC2A4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC2A4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Solute carrier family 2, facilitated glucose transporter member 4 microplate
• Mouse Solute carrier family 2, facilitated glucose transporter member 4 standard
• Mouse Solute carrier family 2, facilitated glucose transporter member 4 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Solute carrier family 2, facilitated glucose transporter member 4 (SLC2A4) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SLC2A4. No significant cross-reactivity or interference between Mouse SLC2A4 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: SLC2A4 is a member of the solute carrier family 2 (facilitated glucose transporter) family and encodes solute carrier family 2, facilitated glucose transporter member 4 that functions as an insulin-regulated facilitative glucose transporter. In the absence of insulin, this integral membrane protein is sequestered within the cells of muscle and adipose tissue. Within minutes of insulin stimulation, the protein moves to the cell surface and begins to transport glucose across the cell membrane. Mutations in SLC2A4 have been associated with noninsulin-dependent diabetes mellitus (NIDDM) .

Alternative Names: SLC2A4; GLUT4; glucose transporter 4; insulin-responsive glucose transporter type 4

Search name: SLC2A4; GLUT4; glucose transporter 4; insulin-responsive glucose transporter type 4

Tag: SLC2A4