Mouse SPD (Surfactant Associated Protein D) ELISA Kit | ELK2953

Mouse SPD (Surfactant Associated Protein D) ELISA Kit | ELK2953 | Gentaur UK, US & Europe Distribution

Alternative Names: SFTPD; COLEC7; PSP-D; SFTP4; SP-D; Pulmonary Surfactant Protein D; Collectin-7; Lung surfactant protein D

Species: Mouse

Assay Type: Sandwich

Sensitivity: 12.7 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: serum, plasma, tissue homogenates, lung lavage fluid and other biological fluids

Assay Lenght: 3.5h

Research Area: Infection immunity;Pulmonology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Surfactant Associated Protein D (SPD) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Surfactant Associated Protein D (SPD) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Surfactant Associated Protein D (SPD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Surfactant Associated Protein D (SPD) in the samples is then determined by comparing the OD of the samples to the standard curve.