Mouse Sphingosine-1-phosphate lyase 1 (SGPL1) ELISA Kit | AE19760MO

Mouse Sphingosine-1-phosphate lyase 1 (SGPL1) ELISA Kit | AE19760MO | Gentaur UK, US & Europe Distribution

Species Reactivity: Mouse (Mus musculus)

Abbreviation: SGPL1

Alternative Name: FLJ13811; KIAA1252; SPL;

Application: ELISA

Range: 0.156-10 ng/mL

Sensitivity: 0.055 ng/mL

Intra-Assay: ≤6.3%

Inter-Assay: ≤8.1%

Recovery: 0, 99

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate SGPL1 in samples. An antibody specific for SGPL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySGPL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SGPL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SGPL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Sphingosine-1-phosphate (SPP) participates in the proliferative signal transduction pathways of mammalian cells. SPP synthesis is catalyzed by sphingosine kinase, and SPP degradation is catalyzed by SPP lyase (SPL) . The first SPL gene cloned, BST1 ('bestowed of sphingosine tolerance'), was isolated from budding yeast . A yeast mutant strain containing a deletion of the entire BST1 coding region is sensitive to the growth-inhibitory effects of D-erythro-sphingosine, due to its inability to degrade SPP. Spl, that has 59% sequence similarity to the BST1 gene product. They showed that this cDNA can functionally complement the yeast BST1 deletion, restoring a sphingosine-resistant phenotype. Northern blot analysis detected Spl expression in several mouse tissues, with the highest level found in the liver.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .