Mouse Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial (SDHD) ELISA Kit | KTE70467

Mouse Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial (SDHD) ELISA Kit | KTE70467 | Gentaur UK, US & Europe Distribution

Application: This Mouse Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial (SDHD) ELISA Kit employs a two-site sandwich ELISA to quantitate SDHD in samples. An antibody specific for SDHD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySDHD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SDHD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SDHD bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial microplate
• Mouse Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial standard
• Mouse Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial (SDHD) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SDHD. No significant cross-reactivity or interference between Mouse SDHD and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: SDHD, which stands for succinate dehydrogenase complex subunit D, is one of the two transmembrane subunits of the four-subunit succinate dehydrogenase protein complex that resides in the inner mitochondrial membrane. It also refers to the gene that codes for this protein. Germline mutations in SDHD were first linked to hereditary paraganglioma in 2000. Since then, it has been shown that mutations in SDHB and to a lesser degree SDHC can cause paranglioma as well familial pheochromocytoma. Notably, the tumor spectrum is different for the different mutations. SDHB mutations often lead to metastatic disease that is extra-adrenal, while SDHD mutation related tumors are more typically benign, originating in the head and neck.

Alternative Names: SDHD; CBT1; PGL; PGL1; SDH4; succinate dehydrogenase complex; subunit D; succinate dehydrogenase ubiquinone cytochrome B small subunit

Search name: SDHD; CBT1; PGL; PGL1; SDH4; succinate dehydrogenase complex; subunit D; succinate dehydrogenase ubiquinone cytochrome B small subunit

Tag: SDHD